Because non-pharmacological methods of pain relief have no side effects on the mother and fetus and are easy to use, they are applicable in the labor process of labor. The present study was conducted to determine the effect of Quran sound on the intensity of pain and other parameters influencing the first stage of labor , as well as the nulliparous women , admitted to Ghamar-Bani Hashem Hospital in Khoi city , Iran. This was a quasi-experimental study conducted on 60 nulliparous women, selected and randomly divided into two groups. Immediately after entry to the delivery room, vital signs were recorded and the intensity of baseline pain was monitored and recorded using Visual Analog Scale. Then, only for the trial group, during the active phase of first stage of delivery, Quran sound (recited by Abdolbaset) was played alternately through headphones for half an hour; then, the intensity of pain and vital signs were measured and recorded. The statistical t-test was used for analysis of data, and the significance level was considered less than 0.05. The majority of subjects were 20-24 years old. The intensity of pain during the active phase of delivery showed a significant differences in the two control and trial groups. It is recommended that the Quran sound be used as a non-pharmacological method for pain relief, shortening the duration of the first stage delivery, improving the vital signs, and increasing the infant’s Apgar score.